Phospho-SMAD 1/5/8 – MGI gene detail for Smad1, 5, 8 Description: Activation of BMP signaling is observed in the nasal mesenchyme of the palatal shelves at E13.5 and E14.5 along the AP axis. A few P-SMAD1/5/8-positive cells in the oral and nasal epithelia are also detectable (not depicted here). Source: Han et al. (2009) Indirect modulation…Continue Reading P-SMAD1/5/8
Year: 2011
Pitx2 gene expression
Pitx2 (Paired-like homeodomain transcription factor 2 / Pituitary homeobox 2) – MGI gene detail Description: At E13.5, Pitx2 is expressed in the palatal epithelium along the AP axis. At E14.5, Pitx2 is expressed in both the epithelium and the osteogenic mesenchyme along the AP axis. Source: Iwata et al. (2012) Fibroblast growth factor 9 (FGF9)-pituitary homeobox 2 (PITX2)…Continue Reading Pitx2 gene expression
Runx2 gene expression
Runx2 (Runt-related transcription factor 2) – MGI gene detail Description: At E13.5 and E15.5, Runx2 is expressed in the osteogenic mesenchyme along the AP axis. Sources: Yamashiro et al. (2002) Expression of Runx1, -2 and -3 during tooth, palate and craniofacial bone development. Mech. Dev. 119 Suppl 1:S107-10. Baek et al. (2011) Bmpr1a signaling plays critical roles in palatal…Continue Reading Runx2 gene expression
Shox2 gene expression
Shox2 (Short stature homeobox gene 2) – MGI gene detail Description: Shox2 is expressed in the mesenchyme of the anterior region of the developing palate, on both nasal and oral sides. Source: Yu et al. (2005) Shox2-deficient mice exhibit a rare type of incomplete clefting of the secondary palate. Development 132(19): 4397-406….Continue Reading Shox2 gene expression
Sox2 gene expression
Sox2 (SRY-box containing 2) – MGI gene detail Description: Sox2 is expressed in the palatal epithelium along the AP axis throughout development, including the oral and nasal epithelia and in the MEE from E14.5 to E15.5. Source: Immunofluorescence and Sox2-GFP knockin mice. Unpublished data from Chai’s lab….Continue Reading Sox2 gene expression
Tgfbr2 gene expression
Tgfbr2 (TGF-Beta receptor 2) – MGI gene detail Description: Tgfbr2 is expressed in both epithelium and mesenchyme along the oral-nasal and anterior-posterior axes at E13.5. Data at other stages is not available. Source: Radioactive in situ hybridization. Unpublished data from Chai’s lab….Continue Reading Tgfbr2 gene expression
Tgfb3 gene expression
Tgfb3 (Transforming growth factor beta 3) – MGI gene detail Description: Tgfb3 is expressed in the midline epithelium before the palatal shelves have contact in the midline (E14.0) until the complete disappearance of the MEE (E15.5). Sources: Yang LT, Li LY & Kaartinen V, 2008. Tissue-specific expression of Cre recombinase from the Tgfb3 locus. Genesis 46(2):112-8. Jackson…Continue Reading Tgfb3 gene expression
Microarray: E14.5 Tgfbr2 Palate
Whole-genome transcriptome profiling by microarray of palatal tissue of E14.5 murine embryos with and without a conditional inactivation of Tgfbr2 in the cranial neural crest See a heatmap of the top 10 most differentially expressed genes between mutant and control samples for the following signaling pathways: BMP/TGF-Beta signaling pathway FGF signaling pathway Hedgehog signaling pathway Wnt…Continue Reading Microarray: E14.5 Tgfbr2 Palate
Wnt1-Cre;Tgfbr2fl/fl mouse palate SEM images
Scanning Electron Microscope (SEM) images (click to enlarge) E18.5 control E18.5 Wnt1-Cre;Tgfbr2 fl/fl…Continue Reading Wnt1-Cre;Tgfbr2fl/fl mouse palate SEM images
Tbx1 gene expression
Tbx1 (T-box 1) – MGI gene detail Description: At E12.5, Tbx1 expression appears in the epithelium of the most posterior domain of the palatal shelf. Tbx1 is expressed in the medial and posterior edge of the secondary palate before and after fusion. Sources: Funato et al. (2012) Tbx1 regulates oral epithelial adhesion and palatal development. Hum. Mol. Genet. 21(11):2524-37….Continue Reading Tbx1 gene expression