Genotype K14-Cre;Tgfbr2fl/fl Symbol (MGI) Tgfbr2tm1.2Hlm Known defects Submucosal cleft palate Known human diseases Histology P0 SEM P0 μCT P0: soft – hard 3D μCT P0: soft – hard μMRI P0: soft Gene expression analysis Protein expression analysis References Iwata J. et al., Development 2013…Continue Reading Craniofacial phenotype analysis: K14-Cre;Tgfbr2fl/fl
Year: 2013
Craniofacial phenotype analysis: Wnt1-Cre;Tgfbr2fl/fl
Genotype Wnt1-Cre;Tgfbr2fl/fl Symbol (MGI) Tgfbr2tm1.2Hlm Known defects Cleft palate, bone defects, small tongue Known human diseases Loeys-Dietz Syndrome Histology E18.5 SEM E18.5 μCT E18.5: soft – hard 3D μCT E18.5: soft – hard μMRI E18.5: soft Gene expression analysis E14.5 Protein expression analysis References Ito Y et al., Development 2003Sasaki T et al., Development 2006Oka K et al., Dev Biol…Continue Reading Craniofacial phenotype analysis: Wnt1-Cre;Tgfbr2fl/fl
Class III cleft palate
Class III: Palatal shelves fail to meet at the midline Complete cleft of secondary palate Ctgf-/- K14-Cre;Shhfl/fl Wnt1-Cre;Alk5fl/fl Wnt1-Cre;Tgfbr2fl/fl Partial cleft of secondary palate: anterior K14-Cre;Alk5fl/fl K14-Cre;Tgfbr2fl/fl Shox2-/- Wnt1-Cre;Shox2fl/fl Partial cleft of secondary palate: posterior / cleft soft palate K14-Cre;Alk5fl/fl K14-Cre;Tgfbr2fl/fl Tbx1-/-…Continue Reading Class III cleft palate
Bmp4 gene expression
Bmp4 (Bone morphogenetic protein 4) – MGI gene detail Description: Bmp4 is expressed in both epithelium and mesenchyme at E12.5. Its expression is restricted to the mesenchyme from E13.5. Bmp4 is expressed only in the anterior and distal regions of the palatal shelves. Source: Zhang et al. (2002) Rescue of cleft palate in Msx1-deficient mice by transgenic…Continue Reading Bmp4 gene expression
Beta-catenin gene expression
Beta-catenin – MGI gene detail Description: Beta-catenin is expressed in the palatal epithelium along the AP axis throughout development, including the oral and nasal epithelia and in the MEE at E14.5. Source: He et al. (2011) Epithelial Wnt/β-catenin signaling regulates palatal shelf fusion through regulation of Tgfβ3 expression. Dev. Biol. 350(2): 511-9….Continue Reading Beta-catenin gene expression
Col III gene expression
Type III Collagen – MGI gene detail Description: Col III is expressed along the AP axis in the nasal and oral mesenchyme at E12.5 and then it is restricted to the oral mesenchyme from E13.5. Source: Immunostaining. Unpublished data from Chai’s lab….Continue Reading Col III gene expression